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Scientist II, Molecular Biology / Biochemistry
Takeda Pharmaceutical in Cambridge, Massachusetts
 
 
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Date Posted September 12, 2017
Category
Science-Biochemistry
Employment Type Fulltime
Application Deadline Open until filled
 
 
 

OBJECTIVES:

The Immunobiologics group within Immunology Research at Takeda Boston is seeking an outstanding candidate with deep and extensive molecular biology and protein biochemistry experience. The role is a lab based research scientist who will work on cutting edge Immuno-oncology platforms in collaboration with internal colleagues within Immunology and Oncology teams as well as external partnerships. The successful candidate will be responsible for designing novel constructs, generating testable hypotheses and executing and interpreting well controlled experiments to advance and augment the Immuno-Oncology pipeline with novel therapeutic modalities such as single domain Abs, cyclic peptides and non Ig scaffolds. The candidate will also lead the development of platforms for small scale expression and purification of the various therapeutic modalities for use in in vitro screening and in vivo research studies. The candidate should also be able to utilize and interpret results using Next Generation sequencing tools, proteomics and molecular approaches to aid the discovery project teams and scientists in delineating molecular mechanisms for immune-oncology applications.

 

ACCOUNTABILITIES:

  • Provide Molecular Biology and Expression Technologies across multiple platform collaborations

  • Manages resource allocation across programs based on needs and prioritization

  • Independently designs, plans and executes experiments to innovate or refine constructs, platform design, expression vectors

  • Implements ideas and provides scientific input for high through put expression of various antibody formats by interfacing with project teams to meet timelines and  blueprints

  • Designs expression vectors for a variety of proteins (mAb, sdAbs, antigen, protein domains, etc…) in both mammalian and non-mammalian expression systems

  • Leads efforts to establish and optimize sdAb expression platform for biologic programs in IO

  • Supporting therapeutic discovery and target validation efforts by developing efficient techniques to generate cell lines expressing desired surface antigens

  • Designs high throughput sequencing liability assessment of sdAbs by interfacing with informatics

  • Interfacing with the Structural Biology in the following areas

    • Assisting in the expression and supply of protein targets to support IO biologics programs

    • Providing scientific input for QC of protein material generated

    • Working in cross-functional teams to harmonize best practices between the groups (where appropriate)

  • Participate in due diligence activities and identify novel technologies for evaluation

Qualifications

EDUCATION, BEHAVIOURAL COMPETENCIES AND SKILLS:

  • PhD in Molecular Biology or Biochemistry with 2 years of postdoctoral training and 2+ years of relevant experience, preferably in industry

  • Publications in peer reviewed journals demonstrating achievement in the above

  • Extensive hands-on experience and knowledge in recombinant DNA and molecular biology techniques including PCR, DNA mutagenesis, RNA isolation, cloning vector design, construct and optimization of expression vectors across a variety of proteins for both mammalian and non-mammalian systems including E.coli, yeast

  • Expertise in transient and stable HEK 293/CHO (preferably CHO-GS) cell line expression for antibody (sdAbs)  production

  • Research experience with CRISPR- based gene editing and cutting edge NGS tools including data analysis is preferred

  • Broad familiarity with novel antibody scaffolds and formats is required

  • Familiarity with flow cytometry and/or sorting is preferred

  • Familiarity with computational modeling tools such as Pymol, Vector NTI, Chimera, Schrodinger preferred

  • Excellent written and verbal communication skills, along with the ability to work in cross-functional project teams

  • Innovative and critical thinking

  • Self-motivated, collaborative with excellent time management and organizational skills

  • Ability to advance multiple projects in a fast paced “biotech like” environment

 

 

PHYSICAL DEMANDS:

  • Manual dexterity required to operate office equipment (i.e., computers, phones, etc.).   

  • Carrying, handling and reaching for objects up to 25 lbs.

  • Working in a lab environment: standing, walking, bending, etc.

TRAVEL REQUIREMENTS:

 

  • Willingness to travel to various meetings or client sites, including overnight trips.  Some international travel may be required.

  • Requires approximately 0-5% travel.

we are driven to improve people's lives

 

www.takedajobs.com

 

Takeda is an EEO employer of minorities, women, disabled, protected veterans. For more information, visithttp://www.takeda.us/careers/EEO_Policy_Statement.aspx

 

No Phone Calls or Recruiters Please.


Full-time

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